Estrogen Receptor alpha Antibody

• Catalog Number: orb1823110
• Category: Antibodies
• Description: Recognizes a protein of 67kDa, which is identified as estrogen receptor (ER) alpha. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. This antibody strongly stains the nucleus of epithelial cells in breast carcinomas.The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to antiestrogen (e.g. tamoxifen) therapy.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rESR1/8761
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A recombinant fragment corresponding to the C-terminus of human ER-alpha was used as the immunogen for the Estrogen Receptor alpha antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Estrogen Receptor alpha antibody is available for research use only.
• UniProt ID: P03372
• Storage: Aliquot the Estrogen Receptor alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human breast carcinoma tissue with Estrogen Receptor alpha antibody (clone rESR1/8761). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Estrogen Receptor alpha antibody (clone rESR1/8761) as confirmation of integrity and purity.