Estrogen Receptor alpha antibody

• Catalog Number: orb1151453
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Estrogen Receptor alpha
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ESR1/4039R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant protein fragment from the C-terminal region was used as the immunogen for the recombinant ERa antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant ERa antibody is available for research use only.
• UniProt ID: P03372
• Storage: Aliquot the recombinant ERa antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant ERa antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human breast carcinoma tissue with recombinant ERa antibody (clone ESR1/4039R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma tissue with recombinant ERa antibody (clone ESR1/4039R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant ERa antibody (clone ESR1/4039R) as confirmation of integrity and purity.