Estrogen Receptor alpha antibody

• Catalog Number: orb1151452
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Estrogen Receptor alpha
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ESR1/6983R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A portion of amino acids 495-595 was used as the immunogen for the recombinant ESR1 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant ESR1 antibody is available for research use only.
• UniProt ID: P03372
• Storage: Aliquot the recombinant ESR1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant ESR1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human MCF-7 cell lysate using recombinant ESR1 antibody (clone ESR1/6983R).

IHC staining of FFPE human breast carcinoma tissue with recombinant ESR1 antibody (clone ESR1/6983R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.