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Catalog Number | orb256527 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Estrogen Recepter beta 2 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Estrogen Receptor beta. The exact sequence is proprietary. |
Dilution range | WB: 1:500-1000, IF/ICC: 1:100-500 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | ESR2 |
Entrez | 13983, 25149, 2100 |
UniProt ID | O08537, Q92731, Q62986 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti ER BETA antibody, anti ER BETA antibody, anti Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Long, Zi et al. Gestational bisphenol A exposure induces fatty liver development in male offspring mice through the inhibition of HNF1b and upregulation of PPARγ Cell Biol. Toxicol., (2020)
Western blot analysis of Estrogen Receptor beta expression in U87MG (A) whole cell lysates. (Predicted band size: 35-59 kD; Observed band size: 59 kD)
Immunofluorescent analysis of Estrogen Receptor beta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
FC, WB | |
Bovine, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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