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Catalog Number | orb1260061 |
---|---|
Category | Antibodies |
Description | ESRRA Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-423 of human ESRRA (NP_004442.3). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 46kDa |
Target | ESRRA |
UniProt ID | P11474 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Steroid hormone receptor ERR1, Estrogen receptor-l Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ESRRA antibody (orb1260061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human uterus using ESRRA antibody (orb1260061) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using ESRRA antibody (orb1260061) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using ESRRA antibody (orb1260061) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ESRRA antibody (orb1260061) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ESRRA antibody (orb1260061) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ESRRA antibody (orb1260061) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using TFAM antibody (orb1260360) at dilution of 1:100 (40x lens).
FC, IF, IHC-P, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |