ESET antibody

• Catalog Number: orb345472
• Category: Antibodies
• Description: ESET antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1050-1075 of human ESET.
• Concentration: 1.17 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: User Optimized, WB: 1:1,000 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human ESET protein.
• Conjugation: Unconjugated
• UniProt ID: Q15047
• NCBI: 224177469
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-ESET antibody, ERG-associated protein
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Western blotting shows reactivity specific for human ESET detecting a band at approximately 170 kDa. Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.
• Expiration Date: 12 months from date of receipt.

Western blot analysis is shown using Biorbyt's Affinity Purified anti-ESET antibody to detect human ESET present in a 293 whole cell lysate (p/n orb348669). ~30 µg of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~170 kDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5000 for 45 min.