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Catalog Number | orb420468 |
---|---|
Category | Antibodies |
Description | ERK1/2 antibody (Peroxidase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Anti-ERK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH. |
Antibody Type | Primary Antibody |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:40,000, WB: 1µg/mL |
Form/Appearance | Lyophilized |
Purity | This HRP conjugated affinity purified antibody is directed against human ERK1 protein. Anti-ERK1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Cross reactivity is expected to occur with human, mouse and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform. |
Conjugation | HRP |
UniProt ID | P27361 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | rabbit anti-ERK-1 Antibody Peroxidase conjugation, Read more... |
Note | For research use only |
Application notes | Anti-ERK 1 (RABBIT) antibody is suitable for use in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43 kDa. |
Expiration Date | 12 months from date of receipt. |