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Catalog Number | orb704524 |
---|---|
Category | Antibodies |
Description | ERK1 antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | 3A12 |
Tested applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
Predicted Reactivity | Mouse, Rat, Zebrafish |
Reactivity | Human |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human ERK1/2 |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:100, IF=1:50-200, Flow-Cyt=1ug/Test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 42/44 kDa |
Target | ERK1/2 |
UniProt ID | Q63844 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | ERK1 + ERK2; ERK 1; ERK 2; ERK-2; ERK1; ERK2; ERT1 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry of Hela.Primary Antibody (green line): Rabbit Anti-ERK1/2 antibody; Dilution:1μg /10^6 cells.
ICC analysis of A549 cell using ERK1/2 monoclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.
IHC-P analysis of rat colon using ERK1 2 Monoclonal Antibody at 1:200 dilution.
IHC-P analysis of mouse brain using ERK1 2 Monoclonal Antibody at 1:200 dilution.
Western blot analysis using Anti-ERK1/2 antibody at 1:1000 dilution.
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, WB | |
Bovine, Canine, Equine, Gallus, Guinea pig, Human, Rabbit, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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