ERBB2 Antibody

• Catalog Number: orb1672075
• Category: Antibodies
• Description: ERBB2 Antibody
• Clonality: Recombinant
• Clone Number: SAIC-02A-7
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Peptide "GLQSLPTHDPSPLQR" derived from the receptor tyrosine kinase erbB2 conjugated to KLH.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: ERBB2
• UniProt ID: P04626
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: HER2, MLN19, NEU, NGL, Receptor tyrosine-protein k
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of MCF7 cells using anti-ERBB2 antibody (SAIC-02A-7). Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric r version of SAIC-02A-7 (orb1672075) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672075- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Flow-cytometry using the anti-ERBB2 antibody SAIC-02A-7. MCF7 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r1 version of SAIC-02A-7 (orb1672075 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.