ERBB2 Antibody

• Catalog Number: orb1671728
• Category: Antibodies
• Description: ERBB2 Antibody
• Clonality: Recombinant
• Clone Number: FWP51
• Tested applications: FC, IF, IP
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: 20x10^6 SKBR3 human breast tumour cells were injected into BALB/c mice. The hybridomas generated from the mice spleen cells were then screen for antibodies reactive to c-erbB-2 using immunofluorescence and immunoprecipitation.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: ERBB2
• UniProt ID: P04626
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD340, Receptor tyrosine-protein kinase erbB-2, Ne
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of MCF7 cells using anti-c-erbB-2 (FWP51). Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric mouse IgG version of FWP51 (orb1671728) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671728- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-c-erbB-2 antibody FWP51. Paraformaldehyde fixed MCF7 cells were stained with anti-unknown specificity antibody (.1; isotype control - black line) or the mouse IgG1 version of FWP51 (orb1671728 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.