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Catalog Number | orb251519 |
---|---|
Category | Antibodies |
Description | ERAB/HSD17B10 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human ERAB recombinant protein (Position: E48-P261). Human ERAB shares 87% and 88% amino acid (aa) sequence identity with mouse and rat ERAB, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 26923 MW |
UniProt ID | Q99714 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | 3-hydroxyacyl-CoA dehydrogenase type-2;1.1.1.35;17 Read more... |
Note | For research use only |
Application notes | WB: The detection limit for ERAB is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A549 cells using anti-ERAB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of A431 cells using anti-ERAB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of ERAB using anti-ERAB antibody.Lane 1:human Jurkat cell; 2:human 293T cell; 3:human HeLa cell; 4:human MOLT4 cell.
IF analysis of ERAB using anti-ERAB antibody. ERAB was detected in immunocytochemical section of U20S cells.
IHC analysis of ERAB using anti-ERAB antibody. ERAB was detected in paraffin-embedded section of Human Mammary Cancer Tissue.
IHC analysis of ERAB using anti-ERAB antibody.ERAB was detected in immunocytochemical section of MCF-7 cell.
IHC analysis of ERAB using anti-ERAB antibody.ERAB was detected in immunocytochemical section of MCF-7 cell.
IHC analysis of ERAB using anti-ERAB antibody.ERAB was detected in immunocytochemical section of HeLa cell.
IHC analysis of ERAB using anti-ERAB antibody.ERAB was detected in immunocytochemical section of SMMC-7721 cell.
FC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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