EPHB2 antibody

• Catalog Number: orb1473923
• Category: Antibodies
• Description: Mouse monoclonal antibody to EPHB2
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human EPHB2. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EPHB2
• Entrez: 2048
• UniProt ID: P29323
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5; Ephrin type-B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EPHB2 expression in EPHB2 protein (A) whole cell lysates. (Predicted band size: 117 kD; Observed band size: 34 kD)

Immunohistochemical analysis of EPHB2 staining in human Skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.