EPHB2 Antibody

• Catalog Number: orb1273579
• Category: Antibodies
• Description: EPHB2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Bovine, Frog, Gallus, Human, Mouse, Rat, Zebrafish
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of Chicken EphrinB. Note: Chicken Tyr317 is the homolog of rat and mouse Tyr328, human Tyr329 and Xenopus Tyr310.
• Concentration: batch dependent
• Dilution range: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, chicken, human, mouse, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 46
• Target: EPHB2
• UniProt ID: P28693
• NCBI: P28693
• Storage: EphrinB antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
• Note: For research use only
• Application notes: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, chicken, human, mouse, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Expiration Date: 12 months from date of receipt.

Western blot of rat testes lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr317 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphrinB is completely eliminated by treatment with lamda-Ptase.