EpCAM (Extracellular domain) antibody

• Catalog Number: orb1294311
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EpCAM antibody (Extracellular domain)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human EpCAM
• Dilution range: Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:400 Immunohistochemistry (Paraffin) 1:100-1:150 Flow Cytometry 1:50-1:200
• Conjugation: Unconjugated
• Target: EpCAM / CD326 (Extracellular domain)
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294311 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr.

Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr.

Product: EpCAM antibody; Catalog: orb1294311; Cell: MCF-7.

Cell: MCF-7 (DAPI: 1-300, Phalloidin: 1-300); EpCAM, (orb1294311): 1-200; Anti-rabbit 594: 1-500; NikonEpi-fluorescence (A-ken) 60x.

Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294311 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.