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Item 1 of 7
Item 1 of 7
EpCAM / CD326 (Intracellular domain)
Catalog Number: orb1294312
| Catalog Number | orb1294312 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to EpCAM antibody (intracellular domain) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Intracellular domain corresponding to Human EpCAM |
| Dilution range | Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:200-1:300 |
| Conjugation | Unconjugated |
| Target | EpCAM / CD326 (Intracellular domain) |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294312 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294312 at 1/200 RT 1hr.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294312 at 1/200 RT 1hr.
All lanes: Anti-EpCAM antibody - at 1/800 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane ws then blocked for an hour before being incubated with orb1294312 overnight at 4°C.
All lanes: Anti-EpCAM antibody - at 1/800 dilution, Lysates/proteins at 60 μg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane ws then blocked for an hour before being incubated with orb1294312 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294312 anti-EpCAM antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Cell: A549; DAPI: 1-300; Phalloidin: 1-300; EpCAM, (orb1294312): 1-200; Anti-rabbit 488: 1-500; NikonEpi-fluorescence (A-ken) 60x.
