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Item 1 of 8
Item 1 of 8
EpCAM / CD326 (Extracellular domain)
Catalog Number: orb1294311
| Catalog Number | orb1294311 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to EpCAM antibody (Extracellular domain) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human EpCAM |
| Dilution range | Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:400 Immunohistochemistry (Paraffin) 1:100-1:150 Flow Cytometry 1:50-1:200 |
| Conjugation | Unconjugated |
| Target | EpCAM / CD326 (Extracellular domain) |
| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294311 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr.
Product: EpCAM antibody; Catalog: orb1294311; Cell: MCF-7.
Cell: MCF-7 (DAPI: 1-300, Phalloidin: 1-300); EpCAM, (orb1294311): 1-200; Anti-rabbit 594: 1-500; NikonEpi-fluorescence (A-ken) 60x.
Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294311 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
