EpCAM Antibody

• Catalog Number: orb750183
• Category: Antibodies
• Description: Ep-CAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: EGP40/1556R
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Human protein was used as the immunogen for the recombinant Ep-CAM antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Ep-CAM antibody is available for research use only.
• UniProt ID: P16422
• Storage: Store the recombinant Ep-CAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the recombinant Ep-CAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human colon carcinoma and recombinant Ep-CAM antibody (clone EGP40/1556R). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/1556R) as confirmation of integrity and purity.

IHC testing of FFPE human ovary tissue and recombinant Ep-CAM antibody (clone EGP40/1556R). Required HIER: steam sections in pH9 EDTA for 10-20 min.

Immunofluorescent staining of human MCF7 cells with recombinant Ep-CAM antibody (clone EGP40/1556R, cyan) and Reddot nuclear stain (red).

Flow cytometry testing of human MCF7 cells with recombinant Ep-CAM antibody (clone EGP40/1556R); Red=isotype control, Blue= recombinant Ep-CAM antibody.