EpCAM Antibody

• Catalog Number: orb749657
• Category: Antibodies
• Description: EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EGP40/837
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the EpCAM antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This EpCAM antibody is available for research use only.
• UniProt ID: P16422
• Storage: Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the EpCAM antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot of HCT116 cell lysate using EpCAM antibody (EGP40/837). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM antibody (green). DAPI was used to stain the cell nuclei (blue).

Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone EGP40/837, green) and Reddot nuclear stain (red).

Flow cytometry testing of human MCF-7 cells. Black: cells alone; Green: isotype control; Red: PE-labeled EpCAM antibody.

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM antibody.

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EpCAM antibody.

SDS-PAGE analysis of purified, BSA-free EpCAM antibody (clone EGP40/837) as confirmation of integrity and purity.