EpCAM Antibody

• Catalog Number: orb749368
• Category: Antibodies
• Description: Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. The VU-1D9 antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VU-1D9
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Small cell lung carcinoma cells were used as the immunogen for this EpCAM antibody VU-1D9.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This EpCAM antibody is available for research use only.
• Entrez: 4072
• Storage: Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EpCAM antibody to be titered up or down for optimal performance.1. Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37oC.; 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.; 3. View the recombinant version of this < a href=../tds/recombinant-ep-cam-antibody-rvu-1d9-v3465>EpCAM antibody.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE colon carcinoma stained with EpCAM antibody (clone VU-1D9).

FACS staining (surface) of HT29 cells using EpCAM antibody (VU-1D9, red) and isotype control (green).

Flow cytometry testing of PFA-fixed human MCF7 cells with EpCAM antibody (clone VU-1D9); Red=isotype control, Blue=CF488-labeled EpCAM antibody.

Flow cytometry testing of human MCF7 cells with (orange) and without (gray) CF555-labeled EpCAM antibody (clone VU-1D9).

Flow cytometry testing of bead-bound exosomes derived from human MCF-7 cells with (orange) and without (gray) CF555-labeled EpCAM antibody (clone VU-1D9).

Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone VU-1D9, green) and Nucspot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free EpCAM antibody (clone VU-1D9) as confirmation of integrity and purity.