You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb640035 |
---|---|
Category | Antibodies |
Description | EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | EGP40/2571R |
Tested applications | FACS, IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A recombinant human partial protein (amino acids 100-224) was used as the immunogen for the EpCAM antibody. The epitope of this mAb has been mapped to between amino acids 202-212. |
Dilution range | Flow cytometry: 1-2ug/million cells in 0.1ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant EpCAM antibody is available for research use only. |
UniProt ID | P16422 |
Storage | Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry testing of human MCF7 cells with recombinant EpCAM antibody (clone EGP40/2571R); Red=isotype control, Blue= recombinant EpCAM antibody.
IHC staining of FFPE human prostate carcinoma with recombinant EpCAM antibody (clone EGP40/2571R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human colon carcinoma with recombinant EpCAM antibody (clone EGP40/2571R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2571R) as confirmation of integrity and purity.
FACS, IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
Filter by Rating