EpCAM antibody

• Catalog Number: orb2276550
• Category: Antibodies
• Description: EpCAM antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-326R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P16422
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Adenocarcinoma-associated Antigen; Cell Surface Gl
• Note: For research use only
• Application notes: Positive Control: Kidney: In kidney, distal tubule cells must show a strong predominantly membranous staining, while at a least moderate predominantly basolateral staining must be seen in the majority of proximal tubules cells and in scattered epithelial cells lining the Bowman capsule. Negative Control: Tonsil: EpCAM staining should be absent in lymphocytes or smooth muscle cells of the vessels. Cellular Localization: Cell Surface & Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

EPCAM immunostaining is lacking in an epitheloid malignant mesothelioma.

In normal kidney EpCAM staining is strong in the distal tubules and moderate in the proximal tubulus predominantly basolateral and in epithelial cells lining the Bowman capsule.

Strong EPCAM staining in an adenocarcinoma of the lung.