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Catalog Number | orb1823850 |
---|---|
Category | Antibodies |
Description | EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, is also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | EGP40/8130R |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | A recombinant human EpCAM fragment from extracellular domain (within amino acids 100-224) was used as the immunogen for the EpCAM antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This EpCAM antibody is available for research use only. |
UniProt ID | P16422 |
Storage | Aliquot the EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human prostate carcinoma tissue with EpCAM antibody (clone EGP40/8130R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC testing of FFPE human kidney tissue with EGP40 antibody (clone EGP40/8130R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC testing of FFPE human tonsil tissue with EGP40 antibody (clone EGP40/8130R) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS, IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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