EpCAM Antibody

• Catalog Number: orb1716206
• Category: Antibodies
• Description: EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rEGP40/7133
• Tested applications: FACS, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant EpCAM antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant EpCAM antibody is available for research use only.
• UniProt ID: P16422
• Storage: Aliquot the recombinant EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon carcinoma tissue with recombinant EpCAM antibody (clone rEGP40/7133). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon tissue with recombinant EpCAM antibody (clone rEGP40/7133). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding.