EpCAM Antibody

• Catalog Number: orb1519811
• Category: Antibodies
• Description: EpCAM Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 264 and C-terminus
• Concentration: 500 µg/ml
• Dilution range: WB - 1:1000; IP - 12 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EpCAM
• UniProt ID: P16422
• NCBI: NP_006197.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: HNPCC8; human epithelial glycoprotein-2; KS 1/4 an
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human EpCAM by western blot. Samples: Whole cell lysate (50 µg) from HeLa, MCF-7, K-562, LNCaP, RKO, Hep-G2, Jurkat, HT-29, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (orb1519811) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPL23.

Detection of human EpCAM by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (orb1519811) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human EpCAM (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (orb1519811) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.