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Catalog Number | orb1151769 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to EpCAM |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rEGP40/826 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant human Ep-CAM protein was used as the immunogen for the recombinant EpCAM antibody. The epitope is known to be between amino acids 27-59. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant EpCAM antibody is available for research use only. |
UniProt ID | P16422 |
Storage | Aliquot the recombinant EpCAM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human prostate carcinoma tissue with recombinant EpCAM antibody (clone rEGP40/826). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma with recombinant EpCAM antibody (clone rEGP40/826). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS, IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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