EpCAM Antibody / Extracellular domain

• Catalog Number: orb750043
• Category: Antibodies
• Description: EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EGP40/1384
• Tested applications: ELISA, IF, IHC-P
• Reactivity: Canine, Feline, Human
• Isotype: Mouse IgG
• Immunogen: A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the EpCAM antibody.
• Dilution range: ELISA: order BSA/sodium azide-free format for coating,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This EpCAM antibody is available for research use only.
• UniProt ID: P16422
• Storage: Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the EpCAM antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human ovarian carcinoma and EpCAM antibody (clone EGP40/1384). Required HIER: steam sections in pH6 citrate buffer for 10-20 min.

Immunofluorescent staining of human MCF-7 cells with EpCAM antibody (green, clone EGP40/1384) and Reddot nuclear stain (red).

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using EpCAM antibody (clone EGP40/1384). These results demonstrate the foremost specificity of the EGP40/1384 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free EpCAM antibody (clone EGP40/1384) as confirmation of integrity and purity.

IHC testing of FFPE dog colon and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.

IHC testing of FFPE dog bladder and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.

IHC testing of FFPE cat small intestine and EpCAM antibody (clone EGP40/1384). HIER: steam sections in pH9 EDTA for 10-20 min.