EpCAM Antibody / Extracellular domain

• Catalog Number: orb376650
• Category: Antibodies
• Description: EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EPM17-2
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human partial recombinant protein (extracellular portion) was used as the immunogen for this anti-EpCAM antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This anti-EpCAM antibody is available for research use only.
• UniProt ID: P16422
• Storage: Store the anti-EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-EpCAM antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of human colorectal carcinoma with anti-EpCAM antibody (clone EPM17-2). Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of human MCF-7 cells with EpCAM antibody (green, clone EPM17-2) and Reddot nuclear stain (red).

Western blot testing of human HCT116 cell lysate with EpCAM antibody (clone EPM17-2). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).