ENTPD2 Antibody (N-term)

• Catalog Number: orb1927979
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB23779
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 53665 Da
• Target: This ENTPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of human ENTPD2.
• UniProt ID: Q9Y5L3
• NCBI: NP_982293.1, NP_001237.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Ectonucleoside triphosphate diphosphohydrolase 2,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ENTPD2 Antibody (N-term) in K562 cell line lysates (35 ug/lane). ENTPD2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ENTPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-ENTPD2 Antibody (N-term) at 1:2000 dilution. Lane 1: H.heart lysate. Lane 2: 293 whole cell lysate. Lane 3: U-87 MG whole cell lysate. Lane 4: H.brain whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ENTPD2 Antibody at 1:1000 dilution. Lane 1: human heart lysate. Lane 2: 293 whole cell lysate. Lane 3: U-87 MG whole cell lysate. Lane 4: human brain lysate. Lane 5: human skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing K562 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.