ENTPD1 Antibody

• Catalog Number: orb1257827
• Category: Antibodies
• Description: ENTPD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ENTPD1 (NP_001157655.1).
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 65kDa
• Target: ENTPD1
• UniProt ID: P49961
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: ATPDase, CD39, NTPDase-1, SPG64, ectonucleoside tr
• Note: For research use only
• Application notes: WB: 1:200 - 1:1000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ENTPD1 antibody (orb1257827) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunofluorescence analysis of Raw264.7 cells using ENTPD1 Polyclonal Antibody (orb1257827) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of THP-1 cells using ENTPD1 Polyclonal Antibody (orb1257827) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of human placenta using ENTPD1 Polyclonal Antibody (orb1257827) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.