Enol Pyruvate Carboxylase antibody

• Catalog Number: orb344821
• Category: Antibodies
• Description: Enol Pyruvate Carboxylase antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Anti-Phospho Enol Pyruvate Carboxylase Antibody was produced by repeated immunizations with maize leaves Phospho-enol-pyruvate Carboxylase.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:10,000 - 1:40,000, IHC: User Optimized, WB: 1:1,000 - 1:4,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize]. Cross reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: B8XPZ2
• NCBI: ACN80021.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Phospho Enol Pyruvate Carboxylase Anti
• Note: For research use only
• Application notes: Anti-Phospho Enol Pyruvate Carboxylase antibody has been tested by western blotting and ELISA is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit Anti-Phospho Enol Pyruvate (PEP) Carboxylase antibody. Lane 1: Phospho Enol Pyruvate (PEP) Carboxylase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Phospho Enol Pyruvate (PEP) Carboxylase primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 100 kDa, 100 kDa for Phospho Enol Pyruvate (PEP) Carboxylase. Other band(s): None.