Enol Pyruvate Carboxylase antibody

• Catalog Number: orb344820
• Category: Antibodies
• Description: Enol Pyruvate Carboxylase antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Anti-Phospho Enol Pyruvate Carboxylase Antibody was produced by repeated immunizations with maize leaves Phospho-enol-pyruvate Carboxylase.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:10,000 - 1:40,000, IHC: User Optimized, WB: 1:1,000 - 1:4,000
• Form/Appearance: Lyophilized
• Purity: Anti-Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize]. Cross reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: B8XPZ2
• NCBI: ACN80021.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Phospho Enol Pyruvate Carboxylase Anti
• Note: For research use only
• Application notes: Anti-Phospho Enol Pyruvate Carboxylase antibody has been tested by western blotting and ELISA is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit Anti-Phospho Enol Pyruvate (PEP) Carboxylase antibody. Lane 1: Phospho Enol Pyruvate (PEP) Carboxylase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Phospho Enol Pyruvate (PEP) Carboxylase primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 100 kDa, 100 kDa for Phospho Enol Pyruvate (PEP) Carboxylase. Other band(s): None.