ENO2 antibody

• Catalog Number: orb12678
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ENO2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 195-434 of human ENO2 (NP_001966.1).
• Dilution range: WB: 1:500-1:2000
• Conjugation: Unconjugated
• MW: 47kDa
• UniProt ID: P09104
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: ENO2, HEL-S-279, NSE, enolase 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using ENO2 Rabbit pAb (orb12678) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using ENO2 Rabbit pAb (orb12678) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Raji cells using ENO2 Rabbit pAb (orb12678) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 600 µg extracts of Mouse brain cells using 3 µg ENO2 antibody (orb12678). Western blot was performed from the immunoprecipitate using ENO2 antibody (orb12678) at a dilution of 1:1000.

Western blot analysis of various lysates using ENO2 Rabbit pAb (orb12678) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.