ENO1 Antibody

• Catalog Number: orb1788430
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG2b,k
• Immunogen: 1-415 aa
• Dilution range: WB: 1:1000, IHC-P: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 47169 Da
• Target: Purified His-tagged ENO1 protein was used to produced this monoclonal antibody.
• UniProt ID: P06733
• NCBI: NP_001188412.1, NP_001419.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ENO1;ENO1L1; MBPB1; MPB1; Alpha-enolase; Alpha-eno
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. kideny section using ENO1 Antibody. Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from MCF-7 cell line, mouse stomach, mouse liver tissue lysate, Hela, HepG2, NIH/3T3, A431 cell line (from left to right), using ENO1 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.