ENH/PDLIM5 Antibody

• Catalog Number: orb1527938
• Category: Antibodies
• Description: ENH/PDLIM5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 546 and C-terminus
• Concentration: 200 µg/ml
• Dilution range: WB - 1:1,000 - 1:2,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ENH/PDLIM5
• UniProt ID: Q96HC4
• NCBI: NP_006448.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: ENH; PDZ and LIM domain protein 5; LIM; L9; enigma
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse ENH/PDLIM5 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-ENH/PDLIM5 antibody orb1527938 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human ENH/PDLIM5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-ENH/PDLIM5 antibody orb1527938 used for IP at 6 µg per reaction.