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Catalog Number | orb389401 |
---|---|
Category | Antibodies |
Description | Emerin/EMD Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Emerin (1-48aa MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRL), different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino ac |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 28994 MW |
UniProt ID | P50402 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Emerin;EMD;EDMD, STA; Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-Emerin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control
WB analysis of Emerin using anti-Emerin antibody.Lane 1:rat skeletal muscle tissue;2:mouse cardiac muscle tissue;3:HELA cell.
IF analysis of Emerin using anti-Emerin antibody.Emerin was detected in paraffin-embedded section of human oesophagus squama cancer tissues.
IF analysis of Emerin using anti-Emerin antibody.Emerin was detected in paraffin-embedded section of human oesophagus squama cancer tissues.
IF analysis of Emerin using anti-Emerin antibody.Emerin was detected in immunocytochemical section of U20S cell.
IF analysis of Emerin using anti-Emerin antibody. Emerin was detected in paraffin-embedded section of human lung cancer tissue.
IHC analysis of Emerin using anti-Emerin antibody.Emerin was detected in paraffin-embedded section of human endometrial carcinoma tissues.
IHC analysis of Emerin using anti-Emerin antibody.Emerin was detected in paraffin-embedded section of human colon cancer tissues.
IHC analysis of Emerin using anti-Emerin antibody.Emerin was detected in paraffin-embedded section of human oesophagus squama cancer tissues.
FC, ICC, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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