ELF5 antibody

• Catalog Number: orb213890
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ELF5
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ELF5. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ELF5
• Entrez: 13711, 2001
• UniProt ID: Q8VDK3, Q9UKW6
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti ESE2 antibody, anti ETS-related transcription
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ELF5 expression in HeLa (A), HuvEc (B), Jurkat (C) whole cell lysates. (Predicted band size: 16; 18; 30; 31 kD; Observed band size: 31 kD)

Immunofluorescent analysis of ELF5 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.