Elastin Rabbit Polyclonal Antibody

• Catalog Number: orb312227
• Category: Antibodies
• Description: Elastin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Elastin (681-786/786aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: ELN
• UniProt ID: P15502
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: alpha Elastin/Tropoelastin; Elastin isoform a; ELN
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Uterus (Mouse) Lysate at 40 ug, Lane 2: Lung (Mouse) Lysate at 40 ug, Lane 3: Liver (Mouse) Lysate at 40 ug, Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 5: Lung (Rat) Lysate at 40 ug, Lane 6: Liver (Rat) Lysate at 40 ug, Lane 7: Testis (Rat) Lysate at 40 ug, Lane 8: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 9: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-Elastin (orb312227) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 66 kD, Observed band size: 68 kD.

Sample: Lung (Mouse) Lysate at 30 ug, Primary: Anti-alpha Elastin/Tropoelastin (orb312227) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution, Predicted band size: 70 kD, Observed band size: 68 kD.

The figure annotation: The blue histogram is unstained cells. The green histogram is cells stained with Rabbit Anti-alpha Elastin/Tropoelastin antibody (orb312227) plus secondary antibody. Controls: Positive control: A549 cells. Isotype control: Cell lines treated with rabbit IgG (orb570250) instead of the primary antibody to confirm that primary antibody binding is specific. Secondary only control: Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (orb868805) to confirm no background signal produced from secondary antibody alone. 1 ug in 100 ul 1X PBS containing 0.5% BSA.