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Catalog Number | orb1258453 |
---|---|
Category | Antibodies |
Description | EIF6 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EIF6 (NP_852133.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 27kDa |
Target | EIF6 |
UniProt ID | P56537 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | CAB, EIF3A, ITGB4BP, b(2)gcn, eIF-6, p27(BBP), p27 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using EIF6 antibody (orb1258453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of MCF-7 cells using EIF6 antibody (orb1258453). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using EIF6 antibody (orb1258453) at dilution of 1:50. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2OS cells using EIF6 antibody (orb1258453) at dilution of 1:50. Blue: DAPI for nuclear staining.
ICC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Sheep, Yeast, Zebrafish | |
Frog, Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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