EIF4EBP1 Antibody

• Catalog Number: orb1240310
• Category: Antibodies
• Description: EIF4EBP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: 4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1.The immunogen is located within the last 50 amino acids of 4E-BP1.
• Concentration: 1 mg/mL
• Dilution range: 4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: EIF4EBP1
• UniProt ID: Q13541
• NCBI: NP_004086
• Storage: 4E-BP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: 4E-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: 4E-BP1 Antibody: BP-1, 4EBP1, 4E-BP1, PHAS-I, Euka
• Note: For research use only
• Application notes: 4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 - 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 µg/mL.

Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 µg/mL.

Immunofluorescence of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 20 µg/ml. Green: 4E-BP1 Antibody (orb1240310) Blue: DAPI staining.