EIF4E antibody

• Catalog Number: orb1151649
• Category: Antibodies
• Description: Mouse monoclonal antibody to EIF4E
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-EIF4E-1D3
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant full-length human EIF4E/Eukaryotic translation initiation factor 4E protein was used as the immunogen for the Eukaryotic translation initiation factor 4E antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Eukaryotic translation initiation factor 4E antibody is available for research use only.
• UniProt ID: P06730
• Storage: Aliquot the Eukaryotic translation initiation factor 4E antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Eukaryotic translation initiation factor 4E antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human MCF-7 cells using Eukaryotic translation initiation factor 4E antibody (green, clone PCRP-EIF4E-1D3) and phalloidin (red).

Immunofluorescent staining of PFA-fixed human HeLa cells using Eukaryotic translation initiation factor 4E antibody (green, clone PCRP-EIF4E-1D3).

FACS staining of PFA-fixed human HeLa cells with Eukaryotic translation initiation factor 4E antibody (blue, clone PCRP-EIF4E-1D3) and isotype control (red).

FACS staining of PFA-fixed human Raji cells with Eukaryotic translation initiation factor 4E antibody (blue, clone PCRP-EIF4E-1D3), and isotype control (red).

FACS staining of PFA-fixed human U-87 cells with Eukaryotic translation initiation factor 4E antibody (blue, clone PCRP-EIF4E-1D3), and isotype control (red).

SDS-PAGE analysis of purified, BSA-free Eukaryotic translation initiation factor 4E antibody (clone PCRP-EIF4E-1D3) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Eukaryotic translation initiation factor 4E antibody (clone PCRP-EIF4E-1D3). These results demonstrate the foremost specificity of the PCRP-EIF4E-1D3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.