EIF4A2 Antibody

• Catalog Number: orb1262327
• Category: Antibodies
• Description: EIF4A2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Monkey, Mouse, Rabbit, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This EIF4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-360 amino acids from the C-terminal region of human EIF4A2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 46 kDa
• Target: EIF4A2
• UniProt ID: Q14240
• NCBI: Q14240
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Eukaryotic initiation factor 4A-II, eIF-4A-II, eIF
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:4000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of EIF4A2 Antibody in K562 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EIF4A2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of EIF4A2 using rabbit polyclonal EIF4A2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4A2 gene.