EIF4A2 Antibody

• Catalog Number: orb1257810
• Category: Antibodies
• Description: EIF4A2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 46kDa
• Target: EIF4A2
• UniProt ID: Q14240
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: EIF4A2, DDX2B, EIF4A, EIF4F, BM-010, eIF4A-II, eIF
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (orb1257810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat brain using EIF4A2 antibody (orb1257810) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using EIF4A2 antibody (orb1257810) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using EIF4A2 antibody (orb1257810) at dilution of 1:100 (40x lens).