EIF2S1 (phospho-S51) antibody

• Catalog Number: orb213882
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EIF2S1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S51 of human EIF2S1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EIF2S1
• Entrez: 13665, 54318, 1965
• UniProt ID: P68101, P05198, Q6ZWX6
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti EIF2A antibody, anti Eukaryotic translation i
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EIF2S1 (Phospho-S51) expression in A549 (A), mouse kidney (B), mouse lung (C) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 38 kD)

Immunohistochemical analysis of EIF2S1 (Phospho-S51) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.