EIF2C2 monoclonal antibody (M01), clone 2E12-1C9

• Catalog Number: orb2291236
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant EIF2C2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2E12-1C9
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1 Kappa
• Immunogen: EIF2C2 (AAH07633.1, 483 a.a. ~ 859 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA
• NCBI: AAH07633.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged EIF2C2 is 0.3 ng/ml as a capture antibody.

EIF2C2 monoclonal antibody (M01), clone 2E12-1C9. Western Blot analysis of EIF2C2 expression in MCF-7.

Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to EIF2C2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]

Western Blot analysis of EIF2C2 expression in transfected 293T cell line by EIF2C2 monoclonal antibody (M01), clone 2E12-1C9. Lane 1: EIF2C2 transfected lysate (Predicted MW: 42.4 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of EIF2C2 over-expressed 293 cell line, cotransfected with EIF2C2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 (Cat # orb2291236). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (68.03 KDa).