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AGO2 Antibody
SKU: orb1256768
Featured
Description
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 47-115 of human EIF2C2 (NP_036286.2). |
| Target | AGO2 |
| Molecular Weight | Observed: 110kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−CASC7, EIF2C2, LINC0098PPD, Q1protein argonaute-2, PAZ Piwi domain protein, argonaute 2, RISC catalytic component, cancer susceptibility candidate 7, cancer susceptibility candidate 7 (non-protein coding), eukaryotic translation initiation factor 2C, 2, long intergenic non-protein coding RNA 98protein slicer
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Western blot analysis of extracts of various cell lines, using AGO2 antibody (orb1256768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody (orb1256768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody (orb1256768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AGO2 antibody (orb1256768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using AGO2 antibody (orb1256768) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using EIF2C2 Polyclonal Antibody (orb1256768) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
AGO2
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
AGO2 Antibody (orb1256768)
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