EIF2B1 antibody

• Catalog Number: orb341384
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EIF2B1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant full length protein of human eIF2B1
• Dilution range: WB: 1:500-1:2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EIF2B1
• Entrez: 1967, 209354
• UniProt ID: Q14232, Q99LC8
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: EIF2BA; Translation initiation factor eIF-2B subun
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of eIF2B1 expression in SHSY5Y (A), MCF7 (B), mouse liver (C), mouse brain (D) whole cell lysates. (Predicted band size: 24; 33 kD; Observed band size: 34 kD)

Immunofluorescent analysis of eIF2B1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).