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Catalog Number | orb1525657 |
---|---|
Category | Antibodies |
Description | eIF2B delta/EIF2B4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 50 and 100 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | eIF2B delta/EIF2B4 |
UniProt ID | Q9UI10 |
NCBI | NP_001029288.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | EIF2B; translation initiation factor eIF-2B subuni Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human eIF2B delta/EIF2B4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF2B delta/EIF2B4 antibody orb1525657 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. eIF2B delta/EIF2B4 was also immunoprecipitated by rabbit anti-eIF2B delta/EIF2B4 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of human eIF2B delta/EIF2B4 by immunohistochemistry. Samples: FFPE sections of human colon carcinoma and breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2B delta/EIF2B4 (Cat. No. orb1525657) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
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