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Catalog Number | orb1260821 |
---|---|
Category | Antibodies |
Description | EIF2AK4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EIF2AK4 (NP_001013725.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 187kDa |
Target | EIF2AK4 |
UniProt ID | Q9P2K8 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | EIF2AK4, GCN2, GCN2 eIF2alpha kinase, GCN2-like pr Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of HepG2 cells, using EIF2AK4 Antibody (orb1260821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF2AK4 antibody (orb1260821) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF2AK4 antibody (orb1260821). Blue: DAPI for nuclear staining.
ELISA, IHC-P | |
Bovine, Canine, Equine, Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
FC | |
Bovine, Canine, Equine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IHC, WB | |
Human, Monkey, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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