EHHADH Antibody (C-term)

• Catalog Number: orb1928381
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22585
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 79495 Da
• Target: This EHHADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human EHHADH.
• UniProt ID: Q08426
• NCBI: NP_001159887.1, NP_001957.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Peroxisomal bifunctional enzyme, PBE, PBFE, Enoyl-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EHHADH Antibody (C-term) in mouse liver (lane 1), kidney (lane 2) tissue lysates (35 ug/lane). EHHADH (arrow) was detected using the purified Pab.

Flow cytometric analysis of HepG2 cells using EHHADH Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with EHHADH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of lysates from mousr kidney, rat kidney and liver tissue (from left to right), using EHHADH Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.