EGFRvIII Rabbit Polyclonal Antibody

• Catalog Number: orb499576
• Category: Antibodies
• Description: EGFRvIII Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human EGFRvIII (321-520/1210aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 100/130 kDa
• Target: EGFR
• UniProt ID: P00533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: GFR vIII; EGFR; Epidermal growth factor receptor v
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-EGFRvIII (orb499576) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100/130 kD, Observed band size: 120 kD.

Sample: Hela (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, Primary: Anti-EGFRvIII (orb499576) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100/130 kD, Observed band size: 120 kD.

Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-EGFRvIII (orb499576) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 100/130 kD, Observed band size: 120 kD.